Small-RNA sequencing libraries with greatly reduced adaptor-dimer background
نویسنده
چکیده
Conventional methods for preparing small-RNA–seq libraries by adaptor ligation generate a significant amount of adaptor dimer, thereby resulting in wasted sequencing reads. These methods also do not capture small 5’-capped and 5’-triphosphorylated RNAs. The ScriptMinerTM small-RNA–seq library preparation technology overcomes these limitations. ScriptMinerTM small-RNA libraries contain greatly reduced amounts of adaptor-dimer when compared to conventional methods and also generate coverage that is characteristic of the entire small-RNA transcriptome.
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